Instructions:
Enter a source DNA sequence
in GenBank format (see example below). Next,
select a target DNA fragment by giving its start and end positions,
and specify the length of the pattern recognized by your
desired restriction enzyme(s). Click on the SUBMIT button.
A second page (the Results
Page) will appear with a list of candidate restriction enzymes
that enclose the target fragment DNA. When you click on its name,
a third page (the Result Details Page) will appear showing
the GenBank sequence with the restriction sites in RED and the
target DNA fragment in BLUE. See also IMPORTANT
NOTES.
Source DNA Sequence:
Target DNA Fragment:
Start Position:
End Position:
Restriction Enzyme:
Length of Recognized Pattern:
IMPORTANT
NOTES:
- If you want to try
a different set of parameters, you must go to the third
page (the Result Details) and click on the button labeled 'Parameter
Page'. Clicking on your browser's BACK button will not always
work (script will still use your old parameters).
- Actually, you can enter
a sequence in free form (e.g. directly type in a sequence of
A, G, T, and C's like ATGTCGATC). The second page (Results Page)
will still give you accurate results. However, the GenePHP
GenBank Sequence Viewer in the third page (Result Details page)
will not work properly. For best results, enter a sequence
in GenBank format, strictly following indentations, spacings,
etc.
Example: First 1000 bases
from the SARS virus
(You may copy and paste this into the large box above. Pls
include spaces before '1' in the first line.)
Source DNA Sequence:
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